Formation of Dynamic γ-H2AX Domains along Broken DNA Strands Is Distinctly Regulated by ATM and MDC1 and Dependent upon H2AX Densities in Chromatin
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چکیده
منابع مشابه
In vivo Analysis of H2AX Phosphorylation Induced by γ-Radiation
ABSTRACT Background and Objectives: Exposure to ionizing radiation in modern societies is inevitable and can cause a variety of adverse health effects such as cancer and birth defects. Therefore, a reliable, repeatable and sensitive method is required for evaluation of radiation exposure. The aim of this study was to determine the amount of hist...
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(Shen et al., 2000). However, it is not known whether the INO80 complex functions in DNA damage repair Waltham, Massachusetts 02454 directly or indirectly through other mechanisms. Here, we show biochemical and genetic evidence that the INO80 complex is directly involved in DNA repair. The Summary key mechanism is through a specific interaction between the INO80 complex and the DNA damage-While...
متن کاملVRK1 chromatin kinase phosphorylates H2AX and is required for foci formation induced by DNA damage
All types of DNA damage cause a local alteration and relaxation of chromatin structure. Sensing and reacting to this initial chromatin alteration is a necessary trigger for any type of DNA damage response (DDR). In this context, chromatin kinases are likely candidates to participate in detection and reaction to a locally altered chromatin as a consequence of DNA damage and, thus, initiate the a...
متن کاملCritical role of lysine 134 methylation on histone H2AX for γ-H2AX production and DNA repair
The presence of phosphorylated histone H2AX (γ-H2AX) is associated with the local activation of DNA-damage repair pathways. Although γ-H2AX deregulation in cancer has previously been reported, the molecular mechanism involved and its relationship with other histone modifications remain largely unknown. Here we find that the histone methyltransferase SUV39H2 methylates histone H2AX on lysine 134...
متن کاملγ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin
DNA double-strand breaks (DSBs) are extremely dangerous lesions with severe consequences for cell survival and the maintenance of genomic stability. In higher eukaryotic cells, DSBs in chromatin promptly initiate the phosphorylation of the histone H2A variant, H2AX, at Serine 139 to generate gamma-H2AX. This phosphorylation event requires the activation of the phosphatidylinositol-3-OH-kinase-l...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2009
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2009.04.012